Chlamydia trachomatis: Methods and Protocols

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,Chlamydia trachomatis. These include techniques to identifyChlamydia trachomatisin patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulateChlamydia trachomatisin molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative, practical, and relevant,Chlamydia trachomatis: Methods and Protocolsserves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.

Edition: 01

Publisher: Springer

Copyright: 2020

Published: 11/19