Molecular Profiling: Methods and Protocols

"Over the past twenty years, scientists have developed and refined laboratory techniques, such as PCR, protein and cDNA microarrays, fluorescent imaging and microdissection, to the point that many of these techniques are now standardized ""kit"" protocols and are performed in secondary school laboratory classrooms. By no means does this indicate a decline in scientific advancement. Quite to the contrary, standardization of protocols and widespread availability of reagents is spurring the next scientific revolution: molecular profiling. The vast array of data generated from genome sequencing and in vitro experiments begs scientists to ask the next logical questions: a) how can we use this information for diagnosis and prognosis, b) how does this information correlate with treatment options, c) how can in vitro experimental information be used in conjunction with in vivo information, and d) how can this information be transferred out of the laboratory to the patient bedside?Molecular profiling utilizes the latest advancements in genomics, proteomics, imaging and bioinformatics to answer these questions by providing a molecular portrait of an individual patientsi disease. This portrait aids in the design of treatment regimens tailored specifically to each patient. Initially it may seem daunting to cover such varied topics in one volume but each discipline contributes an integral element of an individualis molecular profile. The aim of this volume is to describe protocols for the current state of the art in molecular profiling, as well as discuss unique facets of the future for molecular medicine.Although many of the techniques discussed in this volume use commercially available reagents and instrumentation, it is imperative for the user/reader to understand the principles and nuances of these techniques as they are designed for use with irreplaceable human tissue specimens. This volume hopefully will have a broad readership base, so in an attempt to provide basic assay information, we have included basic test principles in the introduction to each analytical chapter as well as providing troubleshooting tips and tricks for the competent scientist.Molecular Profiling covers eight topics in relation to human disease: genomics, proteomics, bioinformatics, imaging, intellectual property, tissue preservation/biobanking, individualized therapy regimens, and grant funding. The first four topics illustrate current laboratory protocols for generating data relevant to m

Pages: 447

Series: Methods in Molecular Biology

Volume: 823

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Edition: 01

Publisher: Springer

Copyright: 2012

Published: 12/11

Notes: A New Edition of this Title is Available