Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis

This book is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.

Pages: 337

Series: Methods in Molecular Biology

Volume: 348

Edition: 01

Publisher: Springer

Copyright: 2006

Published: 07/06

Notes: Print on Demand. Please allow additional time for delivery.